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dcas9 ned  (Addgene inc)


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    Structured Review

    Addgene inc dcas9 ned
    Dcas9 Ned, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+ned/pdCas9-NED+(Plasmid+%23109358)/pmc12177974-43-2-3
    Average 93 stars, based on 5 article reviews
    dcas9 ned - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Functional Validation of the Putative Oncogenic Activity of PLAU
    Article Snippet: Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were kind gifts from Keith Joung (Addgene plasmid #47754 and #43860, respectively), and CRISPRoff-v2.1 (OFF2.1) (DNMT3A/3L-dCas9-KRAB) from Jonathan S Weissman [ ] (Addgene Plasmid #167981). .. We used dCas9-NED (no effector domain) (Addgene plasmid #109358) as negative control [ ]. .. The catalytic domain of human histone methyltransferase PRDM9 was amplified from total cDNA of a testicular cancer cell line, and the catalytic domain of the histone methyltransferase DOT1L from human fibroblasts by Pfu DNA polymerase (Thermo Scientific, Leon-Rot, Germany) as described elsewhere [ ].

    Article Title: Epigenetic editing and epi-drugs: a combination strategy to simultaneously target KDM4 as a novel anticancer approach
    Article Snippet: Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were a kind gift from Keith Joung (Addgene plasmid #47754). .. We used dCas9-NED (Addgene plasmid #109358) as a negative control [ ]. .. The Super Krüppel associated box (KRAB) domain, SKD, was amplified by PCR (Phusion Hot Start II High-Fidelity DNA polymerase, Thermo Fisher Scientific) and introduced into dCas9-NED to create dCas9-SKD [ ].

    Article Title: Epigenetic editing and epi-drugs: a combination strategy to simultaneously target KDM4 as a novel anticancer approach.
    Article Snippet: Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were a kind gift from Keith Joung (Addgene plasmid #47754). .. We used dCas9-NED (Addgene plasmid #109358) as a negative control [62]. .. The Super Krüppel associated box (KRAB) domain, SKD, was amplified by PCR (Phusion Hot Start II High-Fidelity DNA polymerase, Thermo Fisher Scientific) and introduced into dCas9-NED to create dCas9-SKD [63].

    Article Title: Functional Validation of the Putative Oncogenic Activity of PLAU .
    Article Snippet: Plasmids Encoding Transiently Expressed dCas9-Effector Domains Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were kind gifts from Keith Joung (Addgene plasmid #47754 and #43860, respectively), and CRISPRoff-v2.1 (OFF2.1) (DNMT3A/3L-dCas9-KRAB) from Jonathan S Weissman [45] (Addgene Plasmid #167981). .. We used dCas9-NED (no effector domain) (Addgene plasmid #109358) as negative control [46]. .. The catalytic domain of human histone methyltransferase PRDM9 was amplified from total cDNA of a testicular cancer cell line, and the catalytic domain of the histone methyltransferase DOT1L from human fibroblasts by Pfu DNA polymerase (Thermo Scientific, Leon-Rot, Germany) as described elsewhere [41].

    Negative Control:

    Article Title: Functional Validation of the Putative Oncogenic Activity of PLAU
    Article Snippet: Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were kind gifts from Keith Joung (Addgene plasmid #47754 and #43860, respectively), and CRISPRoff-v2.1 (OFF2.1) (DNMT3A/3L-dCas9-KRAB) from Jonathan S Weissman [ ] (Addgene Plasmid #167981). .. We used dCas9-NED (no effector domain) (Addgene plasmid #109358) as negative control [ ]. .. The catalytic domain of human histone methyltransferase PRDM9 was amplified from total cDNA of a testicular cancer cell line, and the catalytic domain of the histone methyltransferase DOT1L from human fibroblasts by Pfu DNA polymerase (Thermo Scientific, Leon-Rot, Germany) as described elsewhere [ ].

    Article Title: Epigenetic editing and epi-drugs: a combination strategy to simultaneously target KDM4 as a novel anticancer approach
    Article Snippet: Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were a kind gift from Keith Joung (Addgene plasmid #47754). .. We used dCas9-NED (Addgene plasmid #109358) as a negative control [ ]. .. The Super Krüppel associated box (KRAB) domain, SKD, was amplified by PCR (Phusion Hot Start II High-Fidelity DNA polymerase, Thermo Fisher Scientific) and introduced into dCas9-NED to create dCas9-SKD [ ].

    Article Title: Epigenetic editing and epi-drugs: a combination strategy to simultaneously target KDM4 as a novel anticancer approach.
    Article Snippet: Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were a kind gift from Keith Joung (Addgene plasmid #47754). .. We used dCas9-NED (Addgene plasmid #109358) as a negative control [62]. .. The Super Krüppel associated box (KRAB) domain, SKD, was amplified by PCR (Phusion Hot Start II High-Fidelity DNA polymerase, Thermo Fisher Scientific) and introduced into dCas9-NED to create dCas9-SKD [63].

    Article Title: Functional Validation of the Putative Oncogenic Activity of PLAU .
    Article Snippet: Plasmids Encoding Transiently Expressed dCas9-Effector Domains Plasmids pMLM3705 (dCas9-VP64) and MLM3636 (sgRNAs) were kind gifts from Keith Joung (Addgene plasmid #47754 and #43860, respectively), and CRISPRoff-v2.1 (OFF2.1) (DNMT3A/3L-dCas9-KRAB) from Jonathan S Weissman [45] (Addgene Plasmid #167981). .. We used dCas9-NED (no effector domain) (Addgene plasmid #109358) as negative control [46]. .. The catalytic domain of human histone methyltransferase PRDM9 was amplified from total cDNA of a testicular cancer cell line, and the catalytic domain of the histone methyltransferase DOT1L from human fibroblasts by Pfu DNA polymerase (Thermo Scientific, Leon-Rot, Germany) as described elsewhere [41].



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